In this paper four species, C. chekiangoleosa, C. grijsi, C. octopetala and C. semiserrata with different cold hardiness were used to determine the electrolyte leakage and MDA content in their leaves after treated with different low temperatures ,1.5℃、-6.5℃、-16℃、- 22℃. Results showed that the plasma membrane permeability became much obviously with the fall of temperature. When the temperature is - 16℃,the electrolyte leakage of C. chekiangoleosa,C. grijsi , C. octopetala increased sharply to 1. 89,1. 33, and 1. 64 times more than that of the - 6.5℃. The electrolyte leakage of C. semiserrata was the most at - 6. 5℃, which was 87.1% more than that of the 1.5℃. MDA content changed regularly with the fall of temperature, raised at first and then declined. When the temperature is - 6.5℃, MDA content of C. semiserrata was the most ,which was 2.42 times of the control ; And when the temperature is - 16℃, MDA content of C. chekiangoleosa,C. grijsi,C. octopetala were the highest and were 75% ,1. 39 times and 1.02 times respectively more than that of the control .